Announcement: We are now accepting BWH DOM core facility vouchers!
The DOM is proposing to award approximately 20 vouchers for $1,000-$3,000 (max $5,000) to support the cost of core services for early-career investigators. Details on voucher program, eligibility, and application instructions can be found here.
Single Cell Core Services And Pricing
Base Services
Rates shown here are for MGB Academic Institutions. All Non-MGB Academic Institutions (44%) and For-Profit Industry (59%) will be charged IDCs
Single-Cell RNA Sequencing
$4400
Single-Cell ATAC Sequencing
$4400
Single-Cell Multiome Sequencing
$7700
Single-Cell FFPE (New!)
$5300
Single-Cell RNA Sequencing-Bulk Discount
$3700
- Baseline cell loading is 10,000 (can go up to 30,000)
- Must submit 6 or more samples within 3 months to be qualified
- Libraries will be stored and sequenced together upon last sample submission
Single-Cell RNA Sequencing-HT (Chromium X)
$6700
- Baseline cell loading is 60,000 (can go up to 120,000)
- Deep-sequencing will be charged when exceed baseline loading
- Samples must be run in pairs with a minimum sample number of 2
Every single cell project will include:
- One free consultation on experimental design and sample preparation (Thursdays 930am)
- 10X reagents and buffer, cell capture & library generation
- One free QC to check cell number and viability
- Sample QC prior to loading and post-library generation
- RNA Sequencing and basic bioinformatic QC & Analysis via CellRanger Pipeline
- Troubleshooting assistance and re-sequencing, if necessary, to achieve at least 20K reads/cell
- Price listing is based on each loading sample
Add-ons Services
CITE-Seq (Custom Panel)
$1000
- Your choice of custom CITE-seq (human/mouse/nuclei) antibodies
- Can be combined with the universal CITE-seq panel
CITE-Seq (Universal Panel)
$1400
- Your choice of Total-seq A, B or C (human/mouse) antibodies
- Includes 1 vial of antibody (stains up to 1 million cells)
Cell Hashing
$2200
- Your choice of Total-seq A, B or C (human/mouse/nuclei) antibodies
- Includes all staining antibodies, reagents and final collection tubes
Immune Cell Repertoire
$1100
- Your choice of either TCR, BCR or a combination of TCR and BCR
- Compatible with 5′ standard and 5′ HT projects
Deep Sequencing
$1000
- Each unit covers an extra 10,000 cells when loading more than baseline
- Total charge is calculated by the actual loading cell number
Start Booking Your Single-Cell Project:
Instructions
- Create an account on the MGB Core Management System
- Internal MGB Users: Use MGB ID
- External Non-MGB Users: MGB CMS Registration Instructions
- If you are a new user, or this is a new experiment, please fill out our Questionnaire and send to SingleCell@bwh.harvard.edu
- Our scientific team will review your Questionnaire and set up a consultation to discuss your experiment
- Upon completion of the consultation:
- Update the Questionnaire with any relevant changes
- Complete the Sequencing Request Form
- Send both forms to SINGLECELL@bwh.harvard.edu
- A core representative will respond to you to schedule your sample submission
Sample Submission Deadline
- All samples must be submitted to the core by no later than 2pm