Flow Cytometry Instruments
BD FACSAria Fusion Cell Sorters
Sterile Biosafety Level II Sorting within HEPA filtered safety cabinet
4-Laser “4L” (16 fluorescent channels)
- 405nm Violet Laser; 488nm Blue Laser; 561nm Yellow Green Laser; 640nm Red Laser
5-Laser “5L” (18 fluorescent channels)
- 355nm UV laser; 405nm Violet laser; 488nm Blue laser; 561nm Yellow Green laser; 640nm Red Laser
AriaFusion Filter Guides for 4L and 5L
SONY ID7000 Spectral Cell Analyzer
Spectral technology, easy-to-use software, and automation
5-Lasers, 147 PMT Detectors
- 355nm UV Laser; 405nm Violet Laser; 488nm Blue Laser; 561nm Yellow Green Laser; 640nm Red Laser
- 10um resolution across the entire detector array
- SONY ID7000 optics guide
Loading option
- A 24-tube rack for 5ml tubes (12*75mm)
- A variety of 96-well plates and 384-well plates
Flow Sorting Considerations
Nozzle & Configuration
Each nozzle, paired with a specific frequency, generates droplets of varying sizes with contain cells of interest that the instrument can isolate and sort out. Please select the nozzle that best suits your cell type and size while also taking into consideration the number of events each configuration can process through per hour.
Note: an event is anything that triggers a response on the cytometer, whether it’s a cell, dead cell, RBC, debris, etc. Efficiency of sorting is based on the flow rate (events/second processed by the instrument) in relation to the sorted population’s overall percentage within the sample. A higher percentage of population of interest within the sample = higher efficiency sort.
We offer three different nozzle sizes:
70 µm Nozzle (70 PSI; ~30 million events per hour)
85 µm Nozzle (45 PSI; 20-30 million events per hour)
100 µm Nozzle (20 PSI; ~10-20 million events per hour)
- Default config for plate sorting & 10X Single Cell sorting
Sort Collection
Sorted cells can be collected into:
- 0.5 – 2 mL Eppendorf Tubes
- 5 mL FACS Tubes
- 15 mL Conical Tubes
- 6, 12, 48, 96, or 384 Well Plates (Culture or PCR)
Please make sure to have buffer or media in your collection tubes
Note: The number of cells that are collected into the tube can range significantly, anywhere between 60-90% from what the sorter has recorded, when counted manually by hemocytometer post-sort. This can especially be the case when collected cells are < 10,000 total. Please take this into consideration when planning your experiments.